Tetramethoxystilbene, a selective CYP1B1 inhibitor, suppresses adipogenesis of C3H10T1/2 pluripotent stem cells
10.3969/j.issn.1673-4254.2015.01.14
- VernacularTitle:四甲氧基二苯乙烯抑制多潜能干细胞C3H10T1/2的脂肪分化
- Author:
Cuifang FAN
1
;
Anna ZHU
;
Tingting HUANG
;
Lu LI
;
Suqing WANG
Author Information
1. 武汉大学 人民医院产科
- Keywords:
tetramethoxystilbene;
CYP1B1 inhibitor;
C3H10T 1/2 cells;
peroxisome proliferator-activated receptor gamma;
adipogenesis
- From:
Journal of Southern Medical University
2015;(1):72-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells. Methods In vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10μg/ml insulin, 2μmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0μg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting. Results Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4. Conclusion TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ.
