Differential expression of bone morphogenetic protein and activin membrane- bound inhibitor in mouse adipose tissues and primary preadipocytes
10.3969/j.issn.1673-4254.2015.01.01
- VernacularTitle:BAMBI在小鼠不同类型脂肪组织发育及脂肪细胞分化过程中的差异表达规律
- Author:
Xiao LUO
1
;
Ru JIA
;
Shuangyu WEI
;
Ting YAO
;
Yuxiang WANG
;
Chang LU
;
Whitehead P JONATHAN
;
Jianqun YAN
Author Information
1. 西安交通大学 医学部生理学与病理生理学系 教育部环境与疾病相关基因重点实验室
- Keywords:
bone morphogenetic protein and activin membrane-bound inhibitor;
adipose tissues;
developmental stages;
primary mouse preadipocytes
- From:
Journal of Southern Medical University
2015;(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression profiles of bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) during the development of mouse adipose tissue. Methods The total RNA was extracted for real-time PCR for amplification of BAMBI mRNA from the suprascapular brown adipose tissue (BAT) and subcutaneous (inguinal) and visceral (gonadal) white adipose tissue (sWAT and vWAT, respectively) of mice at various embryonic and postnatal stages, as well as from isolated primary preadipocytes during differentiation. Results In BAT, BAMBI mRNA levels exhibited a transient increase, peaking at day 0 (D0) and declined thereafter. sWAT and vWAT could be isolated from mice from postnatal D21 onwards, in which BAMBI mRNA levels were the highest and decreased at 8 weeks and 6 months. BAMBI mRNA levels were also significantly reduced in primary preadipocytes isolated from vWAT after induced differentiation. BAMBI mRNA expression level was higher in vWAT than in sWAT and BAT at the same developmental stages. Conclusion BAMBI is differentially expressed in different adipose tissues and developmental stages, which supports the hypothesis that BAMBI plays a pivotal role in the development of adipose tissues.
