Expressions of Bim and ERKs in multidrug-resistant hepatic carcinoma cells
10.3969/j.issn.1673-4254.2014.12.28
- VernacularTitle:Bim和细胞外调节蛋白在肝癌多药耐药细胞中的表达
- Author:
Feng YAN
1
;
Xiaomin WANG
;
Quanming MA
;
Sibo YUAN
;
Nan JIANG
Author Information
1. 厦门大学附属中山医院 胃肠外科
- Keywords:
multidrug resistance;
extracellular signal-regulated kinase 1;
extracellular signal-regulated kinase 2;
extracellular signal-regulated kinase 5;
Bim
- From:
Journal of Southern Medical University
2014;(12):1838-1841
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of extracellular signal-regulated kinase (ERK) and the BH3-only subgroup of Bcl-2 related proteins (Bim) in multidrug-resistant hepatocellular carcinoma (HCC) cells and their association with drug resistance in the cells. Methods The multdrug-resistant HepG-2 cell line was established by treatment with gradually increasing doses of ADM. CCK-8 assay was used to determine the drug sensitivity of the cells, and the expressions of MRP-1, P-gp, ERK1, ERK2, ERK5, and Bim were detected with Western blot. Bim mRNA expression level was measured using quantitative real-time PCR. Results The drug resistance indices to ADM, 5-FU and CDDP was 6.8, 4.10, and 4.5 in HepG-2/ADM cells, respectively. The drug-resistantcells showed marked up-regulation of MRP-1, P-gp, ERK1, ERK2 and ERK5 with down-regulated phosphorylated ERK2 protein expression but no significant changes in phosphorylated ERK1 protein expression to result in a decreased ratio of P-ERK1/2 and P-ERK1/2. Bim mRNA and protein expressions were both decreased in HepG-2/ADM cells. Conclusion ERK and Bim are related to multidrug resistance in HepG-2/ADM cells.
