Pro- inflammatory and anti- inflammatory cytokine responses of peripheral blood mononuclear cells in apparently healthy subjects
10.3969/j.issn.1673-4254.2014.11.07
- VernacularTitle:“看似正常者”外周血单个核细胞炎症因子的表达变化
- Author:
Shanshan GAO
1
;
Shan SHU
;
Lijun WANG
;
Juan ZHOU
;
Zuyi YUAN
Author Information
1. 西安交通大学第一附属医院心内科
- Keywords:
C-reactive protein;
apparently healthy people;
inflammation;
cytokines
- From:
Journal of Southern Medical University
2014;(11):1589-1593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the changes in the expression of pro-inflammatory and anti-inflammatory cytokines in the peripheral blood mononuclear cells (PBMCs) and elucidate the inflammatory status of apparently healthy subjects. Methods Peripheral blood samples (5 ml) were collected after fasting for more than 8 h from 14 healthy control subjects and 14 apparently healthy subjects with elevated serum high-sensitivity CRP (hsCRP) level (≥2.0 mg/L). PBMCs were separated by Ficoll density gradient centrifugation and the total RNA was extracted for real-time quantitative PCR analysis of the inflammatory cytokines, and plasma was separated for ELISA analysis of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) expressions. Results The gene expressions of TNF-α and MCPIP were significantly increased in PBMCs of apparently healthy subjects, while IL-6 and MCP-1 only showed slight elevations;IL-10 expression in PBMCs decreased significantly in apparently healthy subjects as compared to that in the control group. The results of ELISA showed significantly elevated TNF-α level without significant changes of plasma IL-6 level in apparently healthy subjects. Conclusion In apparently healthy subjects with normal lipid levels, chronic low-grade inflammation has occurred shown by elevated expressions of the pro-inflammatory cytokines and lowered expressions of the anti-inflammatory cytokines.
