Effect of electro- acupuncture on hepatic Toll- like receptor 4 and nuclear factor κB expressions in rats with non-alcoholic fatty liver disease
10.3969/j.issn.1673-4254.2014.11.06
- VernacularTitle:电针对非酒精性脂肪性肝病大鼠肝TLR4、NF-κB表达的影响
- Author:
Xiaolin CHEN
1
;
Chenglin TANG
;
Hui XIE
;
Nianzhen TANG
;
Jie GAO
;
Renjian LIU
Author Information
1. 重庆医科大学中医药学院
- Keywords:
non-alcoholic fatty liver disease;
electro-acupuncture;
Toll-like receptor 4;
nuclear factorκB
- From:
Journal of Southern Medical University
2014;(11):1584-1588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electro-acupuncture on Toll-like receptor 4 (TLR4) nuclear factor κB (NF-κB) expressions in the liver of rats with non-alcoholic fatty liver disease (NAFLD). Methods SD rat models of NAFLD induced by feeding high-fat diet for 5 weeks were subjected to early or late intervention with electro-acupuncture at the acupoints Zusanli, Fenglong and Sanyinjiao for 20 min once daily for 21 consecutive days. The changes in serum ALT, AST, TC, and TG levels were determined in the rats and hepatic expressions of TLR4 and NF-κB detected with quantitative fluorescence PCR and Western blotting after treatments. Results Serum ALT, AST, TC, and TG levels and TLR4 and NF-κB levels increased significantly after NAFLD onset (P<0.05), and were markedly reduced by interventions with electro-acupuncture (P<0.05). Conclusion Electro-acupuncture at the acupoints Zusanli, Fenglong and Sanyinjiao can modify blood lipid and hepatic function to produce positive effect on NAFLD possibly by modifying lipid metabolism, lowering expressions of TLR4 and NF-κB in the liver, and reducing the impact of inflammation on NAFLD.
