Amentoflavone induces apoptosis in SW480 human colorectal cancer cells via regulatingβ-catenin and caspase-3 expressions
10.3969/j.issn.1673-4254.2014.07.24
- VernacularTitle:穗花杉双黄酮通过影响caspase-3和β-catenin表达诱导结肠癌细胞SW480凋亡
- Author:
Yu YANG
1
;
Wenjuan XU
;
Kang PENG
;
Xuegang SUN
Author Information
1. 南方医科大学中医药学院分子生物学实验室
- Keywords:
amentoflavone;
SW480;
caspase-3;
β-catenin
- From:
Journal of Southern Medical University
2014;(7):1035-1038
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of β-catenin and caspase-3 in amentoflavone-induced apoptosis of human colorectal cancer SW480 cells. Methods MTT assay was used to detect the viability of SW480 cells exposed to amentoflavone, and flow cytometry was employed to assess the cell apoptosis. Western blotting was performed to determine the protein expressions ofβ-catenin and caspase-3 in the exposed cells. Results Amentoflavone dose-dependently inhibited the viability of SW480 cells, and a high concentration of amentoflavone (150μmol/L) obviously induced apoptosis of the cells. Amentoflavone exposure caused significantly increased expression of caspase-3 and suppressedβ-catenin expression in the cells. Conclusion Amentoflavone-induced apoptosis in SW480 human colorectal cancer cells is associated with altered expressions ofβ-catenin and caspase-3.
