Immunoprotection by Sap2 and dendritic cells against systemic Candida albicans infection in mice
10.3969/j.issn.1673-4254.2014.07.23
- VernacularTitle:Sap2蛋白与树突状细胞对系统性白假丝酵母菌感染的免疫保护性
- Author:
Meilan XU
1
;
Jinxing LIU
Author Information
1. 嘉应学院医学院附属医院检验科
- Keywords:
Sap2;
dendritic cells;
systemic Candida albicans infection;
immunoprotection
- From:
Journal of Southern Medical University
2014;(7):1030-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of combined immunization with recombinant Sap2 and dendritic cells (DCs) against systemic Canddida albicans infection. Methods We constructed a prokaryotic expression vector carrying sap2 of Candida albicans to obtain Sap2 protein. Murine DCs were sensitized by pulsing with Candida albicans spores and rope. Five groups of mice were immunized with recombinant Sap2 protein and sensitized DCs, sensitized DCs, naive DCs, Sap2, or PBS alone for 3 times, and the effect of the immunization against systemic Candida albicans infection were assessed by observing the survival of the mice, detecting the percentages of CD4+and CD8+cells, CFU in the kidney homogenate, and examining renal pathologies. Results Immunization with Sap2 and sensitized DCs and with DCs or Sap2 alone all prolonged the mouse survival and produced obvious effect in renal protection and immune enhancement, but such effects were more obvious with the combined immunization. Conclusion Combined immunization with Sap2 protein and DCs offers strong immunoprotection against systemic Candida albicans infection in mice, which provides experimental evidence for the development of new combined vaccines for immunoprotection.
