Role of endoplasmic reticulum stress pathway in podophyllotoxin nanostructured lipid carriers-induced apoptosis of VK2/E6E7 cells
10.3969/j.issn.1673-4254.2014.06.16
- VernacularTitle:内质网应激途径在鬼臼毒素纳米脂质载体诱导VK2/E6E7细胞凋亡的机制
- Author:
Qi WANG
1
;
Kai HAN
;
Xueya LI
;
Yan XIAO
;
Kang ZENG
Author Information
1. 南方医科大学南方医院皮肤科
- Keywords:
podophyllotoxin nanostructured lipid carriers;
VK2/E6E7 cells;
endoplasmic reticulum stress pathway;
apoptosis
- From:
Journal of Southern Medical University
2014;(6):832-836
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of podophyllotoxin nanostructured lipid carriers (POD-NLC)-induced apoptosis of VK2/E6E7 cells mediated by endoplasmic reticulum stress (ERS). Methods VK2/E6E7 cells cultured in vitro were exposed to 0.125, 0.25, and 0.5 μg/ml POD-NLC or blank NLC for 24 h. The intracellular calcium concentration was measured by laser scanning confocal microscopy (LSCM), and the expression levels of GRP78, GRP94, and calpain2 mRNA and proteins in the cells were detected using RT-PCR and Western blotting. Results Compared with the control cells, the cells exposed to POD-NLC showed a concentration-dependent increase of intracellular calcium concentration (P<0.01), and the differences were statistically significant between different dose groups (P<0.05). RT-PCR and Western blotting showed that POD-NLC up-regulated GRP78, GRP94 and calpain2 mRNA and proteins expressions, whish showed significant differences between blank-NLC and the control groups (P>0.05). Conclusion POD-NLC induces apoptosis of VK2/E6E7 cells possibly by triggering the endoplasmic reticulum stress response.
