Establishment of a hepatic fibrosis model induced by diethylnitrosamine in zebrafish
10.3969/j.issn.1673-4254.2014.06.05
- VernacularTitle:二乙基亚硝胺诱导建立斑马鱼肝纤维化模型
- Author:
Kunyuan WANG
1
;
Li LIU
;
Wencong DAI
;
Xiaohui CHEN
;
Xinchun ZHENG
;
Jinlin HOU
Author Information
1. 南方医科大学南方医院感染科暨肝病中心/南方医科大学南方医院器官衰竭研究国家重点实验室
- Keywords:
zebrafish;
liver fibrosis;
diethylnitrosamine;
animal model
- From:
Journal of Southern Medical University
2014;(6):777-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a zebrafish model of liver fibrosis via diethylnitrosamine (DEN)-induced liver injury. Methods A total of 120 wild-type 3-month-old zebrafish were randomly divided into DEN-treated group and control group. The survival rate and behavioral changes of each group were observed. After treatment with DEN for 2, 4, and 6 weeks, liver index was measured, and liver fibrosis was evaluated with HE staining, Gomori staining and Sirius red staining. Results No obvious behavioral change was observed in DEN-treated group during the experiment. Compared with that in control group, the liver index of zebrafish in DEN-treated group showed no significantly changes at the time points of observation. Proliferation of reticulate fibers was found in 30%of zebrafish treated with DEN for 4 weeks, and the rate increased to 80%at 6 weeks when reticulate fibers and collagen fibers actively proliferated to result in fiber collapse and formation of fibrotic nodules. Conclusion A stable zebrafish liver fibrosis model was successfully established by inducing liver damage to facilitate studies of the pathogenesis of liver fibrosis and screening therapeutic drugs.
