Ultra-high performance liquid chromatography-mass spectrometry for analysis of newborn and fetal bovine serum components
10.3969/j.issn.1673-4254.2014.05.34
- VernacularTitle:超高效液相色谱-质谱联用技术分析新生牛和胎牛血清组分
- Author:
Caixia LI
1
;
Fuke WANG
;
Liu LIU
Author Information
1. 昆明医科大学
- Keywords:
ultra-high performance liquid chromatography;
liquid chromatography/mass spectrometry;
newborn calf;
fetal bovine;
serum
- From:
Journal of Southern Medical University
2014;(5):751-753
- CountryChina
- Language:Chinese
-
Abstract:
We used ultra- high performance liquid chromatography- mass spectrometry (UPLC/MS) for analyzing and identifying the active components of newborn calf serum (NCS) and fetal bovine serum (FBS). The results demonstrated significant differences in the components between NCS and FBS. FBS appeared to have more complex components than NCS, with mass to ratios (m/z) of the substances of 498, 273 and 448. These substances in FBS may be the main active components to support the proliferation and differentiation of cells.