Effect of hydrogen sulfide on human colon cancer SW480 cell proliferation and migration in vitro

VernacularTitle:硫化氢对人结肠癌细胞增殖和迁移的影响
Author: Min HONG ¹ ; Xiaoqing TANG ; Kui HE
Author Information

1. 南方医科大学
Keywords: hydrogen sulfide; human colon cancer; SW480; proliferation; migration
From: Journal of Southern Medical University 2014;(5):699-703
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of hydrogen sulfide (H2S) on the proliferation and migration of human colon cancer SW480 cells and explore its molecular mechanisms. Methods The proliferation of SW480 cells exposed to different concentrations of NaHS for varying time lengths was analyzed by MTT assay, and the changes in cell migration was evaluated using wound-healing assay. The changes in the expression levels of MMP-2, MMP-9 and SIRT1 protein were detected by Western blotting in the exposed cells. Results Compared with the control cells, SW480 cells exposed to 50, 100, 200, or 400μmol/L NaHS for 24, 48 and 72 h all showed increased proliferative activity. NaHS treatment at 100 μmol/L significantly promoted the cell migration (P<0.01) and enhanced the cellular expressions of MMP-2 (P<0.05) and MMP-9 (P<0.01) proteins;NaHS exposure (100μmol/L) also resulted in up-regulation of SIRT1 expression in SW480 cells. Conclusion H2S can promote proliferation and migration of SW480 cells in vitro, the mechanism of which may involve up-regulated expression of SIRT1.