Effect of quercetin on glioma cell U87 apoptosis and feedback regulation of MDM2-p53

VernacularTitle:槲皮素诱导U87细胞凋亡及对MDM2-p53负反馈调节的影响
Author: Huili WANG ¹ ; Zhaohu YUAN ; Zhijiang CHEN ; Fang YAO ; Ziyou HU ; Bingyi WU
Author Information

1. 南方医科大学南方医院临床医学实验研究中心
Keywords: quercetin; U87 cells; apoptosis; MDM2-p53
From: Journal of Southern Medical University 2014;(5):686-689
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of quercetin on apoptosis and feedback regulation of MDM2-p53 in multiform glioblastoma U87 cells in vitro. Methods U87 cells exposed to different concentrations of quercetin (50, 100, and 150μmol/L) were examined with flow cytometry, RT-PCR and Western blotting for detecting the cell apoptosis, MDM2 mRNA expression, and p53 and caspase-3 expressions. Results Quercetin induced obvious apoptosis in U87 cells in a concentration-dependent manner, with apoptosis rates of (12.40 ± 0.70)% at Q0, (22.53 ± 0.72)% at Q50, (29.06 ± 0.81)% at Q10 , and (31.5 ± 0.45)% at Q150. Quercetin significantly increased the expressions of MDM2 mRNA and active caspase-3 protein but decreased the expression of p53 in the cells. Conclusion Quercetin promotes the apoptosis of multiform glioblastoma U87 cells mediated by caspase-3 and influences the feedback balance of MDM2-p53.