Advanced oxidation protein products induce epithelial- to- mesenchymal transition in cultured human proximal tubular epithelial cells via oxidative stress
10.3969/j.issn.1673-4254.2014.05.13
- VernacularTitle:晚期蛋白氧化产物通过氧化应激诱导肾小管上皮细胞转分化
- Author:
Jun ZHANG
1
;
Minzi QIU
;
Yaqiong MA
;
Yang BU
;
Lei YANG
;
Xun TANG
Author Information
1. 南方医科大学珠江医院肾内科
- Keywords:
advanced oxidation protein products;
epithelial-to-mesenchymal transition;
human proximal tubular epithelial cells;
HK-2 cells;
oxidative stress
- From:
Journal of Southern Medical University
2014;(5):659-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of advanced oxidation protein products (AOPP) on epithelial-to-mesenchymal transition (EMT) in cultured human proximal tubular epithelial cells (HK-2) and explore the mechanism. Methods HK-2 cells treated with 50, 100, 200, and 400μg/ml AOPP or 50μg/m bovine serum albumin (BSA) for 24 h, or with 200μg/ml AOPP for 0.5, 1, 3, 6, 12, and 24 h were examined for the protein expression of α-SMA and E-cadherin. In cells pretreated with diphenyleneiodonium (DPI) or cytoplasmic superoxide dismutase (C-SOD), the effects of 50μg/ml BSA and 200μg/ml AOPP were assessed on the expressions of α-SMA and E-cadherin, malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, catalase (CAT) activity, and glutathione peroxidase (GSH-px) activity. Results AOPP treatment up-regulatedα-SMA expression and down-regulated E-cadherin expression in a dose- and time-dependent fashion. AOPP exposure of the cells resulted in increased MDA level and lowered activities of SOD, CAT and GSH-PX. DPI and C-SOD partially attenuated the effects of AOPP onα-SMA, E-cadherin, MDA, SOD, CAT and GSH-px. Conclusion AOPP can induce EMT in cultured HK-2 cells via oxidative stress, and this effect can be attenuated by inhibiting the activation of NADPH oxidase and using antioxidants to delay the progression of renal interstitial fibrosis.
