Role of Sam68 in proliferation, invasion and migration of colorectal cancer cells in vitro
10.3969/j.issn.1673-4254.2014.04.23
- VernacularTitle:Sam68对结直肠癌细胞增殖、侵袭和迁移能力的影响
- Author:
Liyang WANG
1
;
Yanmei CUI
;
Wenting LIAO
;
Side LIU
Author Information
1. 南方医科大学南方医院消化内科
- Keywords:
Sam68;
colorectal cancer;
proliferation;
invasion;
migration
- From:
Journal of Southern Medical University
2014;(4):546-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and development of colorectal cancer. Methods Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68 expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using colony formation, MTT and Transwell assays. Results SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05). Conclusion The expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.
