SCF/c-Kit signaling promotes invasion of T24 cells via PI3K pathway

VernacularTitle:SCF/c-Kit信号促进膀胱癌T24细胞侵袭作用
Author: Shujun GUO ¹ ; Xiangnan TAO ; Yimeng WANG ; Jie TANG ; Lin SHEN ; Chuanwang SONG
Author Information

1. 蚌埠医学院免疫学教研室/安徽省感染与免疫重点实验室
Keywords: SCF/c-Kit signaling; T24 cells; invasion; bladder cancer
From: Journal of Southern Medical University 2014;(4):507-510
CountryChina
Language:Chinese
Abstract: Objective To explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells. Methods Western blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24 cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was evaluated using Transwell invasion assay (direct and indirect counting methods). Results T24 cells expressed c-Kit protein and showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect was blocked by the application of Wortmannin before the stimulation. Conclusion SCF/c-Kit signaling promotes the invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.