Effect of exogenous hydrogen sulfide on BACE-1 enzyme expression and β-amyloid peptide metabolism in high-glucose primary neuronal culture
10.3969/j.issn.1673-4254.2014.04.14
- VernacularTitle:外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响
- Author:
Lijuan ZHU
1
;
Xiaoshan CHEN
;
Xuanli HE
;
Yunwen QI
;
Yong YAN
Author Information
1. 重庆医科大学附属第一医院神经内科
- Keywords:
hydrogen sulfide;
primary culture neuron;
high glucose environment;
β-site APP cleaving enzyme 1;
β-amyloid peptide metabolism
- From:
Journal of Southern Medical University
2014;(4):504-506,510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of exogenous hydrogen sulfide (H2S) onβ-site APP cleaving enzyme 1 (BACE-1) and β-amyloid peptide (Aβ) metabolism in primary culture of neurons under high-glucose condition. Methods The cortical neurons in primary culture under normal and high glucose (60 mmol/L) conditions for 24 h were exposed to 25, 50 and 100μmol/L NaHS. Aβ1-42 concentration in the cell culture was measured by ELISA, and BACE-1 mRNA and protein levels were detectedby fluorescent quantitative real-time PCR and Western blotting, respectively. Results Compared with the neurons cultured in normal glucose, the neurons exposed to high glucose showed significantly increased Aβ1-42 concentration and BACE-1 mRNA and protein expressions (P<0.05). Exposure to 25, 50 and 100 μmol/L NaHS significantly decreased Aβ1-42 concentration and BACE-1 mRNA and protein expressions in the high-glucose cell culture (P<0.05). Conclusion Neurons exposed to high glucose exhibit increased Aβ1-42 levels and BACE-1 mRNA and protein expressions, which can be concentration-dependently decreased by NaHS.
