Changes of hypoxia-inducible factor in residual hepatocellular carcinoma following high-intensity focused ultrasound exposure in nude mice
10.3969/j.issn.1673-4254.2014.04.05
- VernacularTitle:高强度聚焦超声治疗后残余肝癌中缺氧诱导因子的变化
- Author:
Zhihao FU
1
;
Lun WU
;
Zhengrong QIAO
;
Shiji ZHOU
;
Shengwei LI
Author Information
1. 重庆医科大学附属第二医院肝胆外科
- Keywords:
liver neoplasms;
hypoxia-inducible factor;
high-intensity focused ultrasound;
residual tumor
- From:
Journal of Southern Medical University
2014;(4):463-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes in hypoxia-inducible factor (HIF1α, HIF2α) in the residual tumor cells in nude mice bearing hepatocellular carcinoma (HCC) following treatment with high-intensity focused ultrasound (HIFU). Methods Thirty nude mice bearing human HCC received treatment with HIFU. At 1, 3, and 5 days and 1 and 2 weeks after the treatment, the mice were examined for pathological changes of the residual tumor with HE staining; SP immunohistochemistry, Western blotting and real-time quantitative PCR were used to detect the protein and mRNA expressions of HIF1α and HIF2α in the tumor. Results HE staining revealed the presence of residual tumor cells and large necrotic areas after the treatment. Immunohistochemistry showed a gradual increment of HIF1αprotein and mRNA expressions after the treatment, reaching the peak level at 3 days (P<0.05) followed by progressive reduction at 5 days and 1 and 2 weeks. HIF2αexpressions at either the protein or mRNA levels exhibited no significant changes within 3 days after the treatment (P>0.05) but increased significantly at 5 days and 1 and 2 weeks (P<0.05). Conclusion The changes of HIF1αand HIF2αin the residual tumor after HIFU treatment in nude mice bearing HCC can be associated with tumor cell apoptosis and angiogenesis after the treatment.
