Effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm
- VernacularTitle:抗菌肽LL-37对鲍曼不动杆菌生物膜的抑制作用
- Author:
Pengwei SHI
1
;
Yanbin GAO
;
Zhiyang LU
;
Lei YANG
Author Information
1. 南方医科大学南方医院烧伤科
- Keywords:
antibacterial peptide;
LL-37;
biofilm;
Acinetobacter baumannii
- From:
Journal of Southern Medical University
2014;(3):426-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm. Methods A model of Acinetobacter baumannii biofilm in vitro was constructed by plates and crystal violet staining method, and the minimal inhibitory concentration of LL-37 was determined by broth dilution. The biofilm morphology was observed by scanning electron microscopy and biofilm formation was analyzed by the crystal violet staining of the adherent biofilm in the presence of antibacterial peptide LL-37. Results In the Acinetobacter baumannii biofilm model, the minimal inhibitory concentration of LL-37 was 64μg/ml;LL-37 caused structural damage of the biofilm at a low concentration of 2.5μg/ml. The biofilm was decreased gradually as the concentration of LL-37 increased. Conclusion LL-37 even at a concentration far below its minimal inhibitory concentration can cause structural damage of Acinetobacter baumannii biofilm in vitro.
