miR- 520a regulates ErbB4 expression and suppresses proliferation and invasion of esophageal squamous cell carcinoma
- VernacularTitle:miR-520a调控ErbB4的表达并抑制食管鳞癌细胞的增殖与侵袭
- Author:
Wenguang YE
1
;
Qinglin YAO
;
Mingxin ZHANG
;
Qinsheng WEN
;
Jingjie WANG
Author Information
1. 第四军医大学唐都医院消化科
- Keywords:
miR-520a;
ErbB4;
esophageal squamous cell carcinoma;
proliferation;
invasion
- From:
Journal of Southern Medical University
2014;(2):164-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of miR-520a in regulation ErbB4 expression and the biological behavior of esophageal squamous cell carcinoma (ESCC). Methods The role of miR-520a in regulating the expression of ErbB4 was investigated by Western blotting and luciferase reporter assay system. The effect of miR-520a on the proliferation and invasion of ESCC cells was detected by MTT and Transwell invasion assay, respectively. Results Western blotting and luciferase reporter assay revealed that miR-520a down-regulated the expression of ErbB4 in vitro. miR-520a significantly inhibited the proliferation and suppressed the invasion of ESCC cell line Eca109. Conclusion miR-520a regulates the expression of ErbB4 and suppresses the proliferation and invasion of ESCC cells in vitro, suggesting its role as a tumor suppressor.
