miR-124a promotes neurite outgrowth by inhibiting iASPP expression
- VernacularTitle:miR-124a通过抑制iASPP基因表达促进神经突起生长
- Author:
Lifang LIN
1
;
Xi GU
;
Shuhu LIU
;
Xuemin WANG
Author Information
1. 南方医科大学基础医学院神经生物学教研室
- Keywords:
miR-124a;
iASPP;
neural development;
neurite outgrowth
- From:
Journal of Southern Medical University
2014;(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of iASPP as the target gene of miR-124a in neural development. Methods Using the online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene. We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques. Results miR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3'UTR of iASPP gene. miR-124a promoted neurite outgrowth of the cells, which was blocked by iASPP overexpression. Conclusion miR-124a promotes neurite outgrowth of M17 cells by inhibiting iASPP expression.
