Effects of beraprost sodium on extracellular matrix metabolism in cultured rat mesangial cells induced by high glucose
10.3969/j.issn.1673-4254.2013.12.24
- VernacularTitle:贝前列素钠对高糖条件下大鼠系膜细胞细胞外基质代谢的影响
- Author:
Qian ZHANG
1
;
Yaoming XUE
;
Yuan YUAN
;
Yingjuan JIANG
;
Ling WANG
Author Information
1. 南方医科大学南方医院内分泌代谢科
- Keywords:
beraprost sodium;
rat mesangial cells;
extracellular matrix;
high glucose
- From:
Journal of Southern Medical University
2013;(12):1819-1822
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effects of beraprost sodium (BPS) on the metabolism of extracellular matrix (ECM) in rat mesangial cells cultured in the presence of high glucose and the possible mechanism. Methods Rat mesangial cells were cultured in the presence of high glucose with or without BPS for 24 or 48 h. The levels of transforming growth factorβ1 (TGFβ1), fibronectin (FN) and matrix metalloproteinase-2 (MMP-2) protein in the culture supernatants were measured by enzyme-linked immunosorbent assay, and photoshop-Smad3 was detected by Western blotting. Results Compared with the cells in normal glucose, the cells cultured in the presence of high glucose for 24 and 48 h showed significantly increased TGFβ1 and FN protein expression and lowered MMP-2 protein expression (P<0.01). Compared with the cells cultured in high glucose, BPS exposure at the concentration of 1, 2, and 5 μmol/L for 24 and 48 h significantly lowered TGFβ1 protein expression (P<0.01), and at 2 and 5 μmol/L, BPS significantly decreased FN protein expression and increased MMP-2 protein expression in high glucose-induced cells (P<0.05). High glucose exposure also significantly increased the expression phosphorylated Smad3 (P<0.01), which was lowered by BPS treatment at 2 and 5μmol/L (P<0.01). Conclusion BPS can regulate ECM metabolism in rat mesangial cells cultured in high glucose by inhibiting TGFβ1/Smad3 pathway, suggesting the beneficial effects of BPS in preventing and treating diabetic nephropathy.
