Effects of Epstein- Barr virus and cytomegalovirus infection on childhood acute lymphoblastic leukemia gene methylation
- VernacularTitle:EB病毒和人巨细胞病毒感染对儿童急性淋巴细胞白血病PTEN及hTERT基因甲基化的影响
- Author:
Xinkun QI
1
;
Yi SHU
;
Ru QIN
;
Lin ZOU
Author Information
1. 重庆医科大学附属儿童医院临床分子医学中心//儿童发育疾病研究省部共建教育部重点实验室//儿科学重庆市重点实验室//重庆市干细胞治疗工程技术研究中心
- Keywords:
Epstein-Barr virus;
human cytomegalovirus;
DNA methylation;
acute lymphoblastic leukemia
- From:
Journal of Southern Medical University
2013;(11):1678-1681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the associations between Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) infections and the methylation levels of PTEN and hTERT genes and explore their roles in children with acute lymphoblastic leukemia (ALL). Methods Blood samples from 100 children with newly diagnosed acute lymphoblastic leukemia were centrifuged for serological detection of EBV and HCMV, and the patients were divided accordingly into EBV-infected group (n=20), HCMV-infected group (n=14), EBV and HCMV co-infected group (n=41), and non-infected group (control group, n=15). DNA was extracted from peripheral blood mononuclear cells (PBMCs) and modified with bisulfite ammonia sodium. The methylation levels of the promoters of PTEN and hTERT genes were detected with methylation-specific polymerase chain reaction (MS-PCR). Results Compared with those in non-infected group and EBV-or HCMV-infected group, the methylation levels of PTEN gene in the co-infected group were significantly decreased (P<0.05) while the methylation levels of hTERT gene significantly increased (P<0.05). Conclusion In children with acute lymphoblastic leukemia, EBV and HCMV co-infection cause changes in the methylation levels of PTEN and hTERT. These results may be associated with epigenetic changes caused by viral infections, and further studies are needed to further verify this hypothesis.