Effect of piperphentonamine hydrochloride on expressions of interleukin-1βand TNF-αmRNA and Fas protein in guinea pigs with cochlear ischemia/reperfusion injury
- VernacularTitle:盐酸椒苯酮胺对耳蜗缺血再灌注后白介素-1β、肿瘤坏死因子-αmRNA及Fas蛋白表达的影响
- Author:
Yonghe LI
1
;
Wei LI
;
Jian WU
;
Hao CHEN
;
Liangcai WAN
Author Information
1. 南方医科大学珠江医院耳鼻咽喉-头颈外科
- Keywords:
ischemia reperfusion;
PPTA;
IL-1β;
TNF-α;
Fas protein
- From:
Journal of Southern Medical University
2013;(11):1669-1672,1677
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between IL-1βand TNF-αmRNA and Fas protein expressions and cochlear ischemia reperfusion injury and investigate the protective mechanism of PPTA against cochlear reperfusion injury. Methods Sixty-four guinea pigs were randomly divided into normal control group, blank control group, ischemia/reperfusion (by clamping the bilateral vertebral artery and right common carotid artery for 1 h) control group, and ischemia/reperfusion with PPTA treatment group. In PPTA group, PPTA was injected via the femoral vein immediately after reperfusion, and ischemia/reperfusion control group received saline injection. In 6 guinea pigs from each group, the cochlear tissues were removed after 24 h of reperfusion for examination of expressions of IL-1β and TNF-α mRNA by real-time PCR, and the rest animals were used for immunohistochemical detection of Fas protein. Results Compared with those of normal group and blank control group, the expressions of IL-1β and TNF-α mRNA increased significantly after cochlear ischemia/reperfusion (P<0.001), but were lowered significantly by PPTA (P<0.001). Positive expression of Fas protein expression was detected in the Corti organ, spiral ganglion and stria vascularis in ischemia/reperfusion control group with significantly higher IOD values than those of the other 3 groups (P<0.05). The IOD value showed no significant difference between PPTA-treated group, normal control group, and blank control group (P>0.05). Conclusions PPTA can suppress the expression of Fas protein and IL-1βand TNF-αmRNAs in the cochlea of guinea pigs with cochlear ischemia/reperfusion. The protective effect of PPTA against cochlear ischemia/reperfusion is mediated probably by inhibition of inflammatory responses and cell apoptosis.
