Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB
- VernacularTitle:电穿孔技术对DNA疫苗pVAX-tG250FcGB免疫效果的影响
- Author:
Yi XIAO
1
;
Kun GAO
;
Yong YANG
;
Jinqi YAN
;
Liang ZHANG
;
Yu WANG
;
Yuanji XU
;
Renli TIAN
;
Zhiyan DU
;
Jiyun YU
Author Information
1. 中国人民解放军总医院第一附属医院泌尿外科
- Keywords:
kidney neoplasms;
DNA vaccine;
electroporation;
immune effect
- From:
Journal of Southern Medical University
2013;(11):1628-1631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX-tG250FcGB. Methods The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared. Results The vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection. Conclusion Electroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.