Carvacrol pretreatment attenuates myocardial oxidative stress and apoptosis following myocardial ischemia-reperfusion in mice
- VernacularTitle:香芹酚预处理减轻小鼠心肌缺血再灌注时氧化应激及细胞凋亡
- Author:
Xudong SONG
1
;
Aihua CHEN
;
Yingfeng LIU
;
Xianbao WANG
;
Yijun ZHOU
;
Lei LIU
;
Xiuli ZHANG
;
Lizi WANG
;
Pingzhen YANG
Author Information
1. 南方医科大学珠江医院心内科电生理与起搏治疗中心
- Keywords:
carvacrol;
heart;
ischemia-reperfusion injury;
oxidative stress;
apoptosis
- From:
Journal of Southern Medical University
2013;(11):1624-1627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of carvacrol pretreatment on myocardial ischemia-reperfusion (I/R) injury and its underlying mechanisms. Methods Wild-type male C57 BL/6 mice were randomized into 5 groups (n=13), namely the sham-operated group, vehicle (DMSO in saline)+I/R group, carvacrol (20 mg/kg)+I/R group, carvacrol (40 mg/kg)+I/R group, and carvacrol (60 mg/kg)+I/R group. The mouse models of myocardial I/R injury were established by a 45-min occlusion of the left anterior descending coronary artery (LAD) followed by reperfusion for 2 h. Carvacrol or vehicle was administered intravenously 15 min before LAD occlusion. After reperfusion, the mice were examined for myocardial oxidative stress level and apoptosis rate. Results Compared with the vehicle group, the 3 carvacrol-pretreated groups showed significantly reduced myocardial infarct size, oxidative stress level and cardiac myocyte apoptosis rate (P<0.01). Conclusion Carvacrol can protect against myocardial I/R injury by inhibiting myocardial oxidative stress and apoptosis in mice.
