Isolation and expression profiling of transformer 2 gene in Aedes aegypti
- VernacularTitle:埃及伊蚊性别决定基因Transformer 2的鉴定与表达分析
- Author:
Peiwen LIU
1
;
Yuting CHEN
;
Jinbao GU
;
Xiaoguang CHEN
Author Information
1. 南方医科大学公共卫生与热带医学学院病原生物学系
- Keywords:
Aedes aegypti;
transformer2;
sex-determining gene;
release of insects carrying a dominant lethal
- From:
Journal of Southern Medical University
2013;(11):1583-1589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate, identify and analyze the sex-determining gene Transformer 2 (Aaetra2) of the major vector mosquito Aedes aegypti. Methods tBLASTn program, RT-PCR and RACE methods were used to obtain the full-length cDNA of Aaetra2. Multiple alignments of nucleotide and amino acid sequences were conducted, and the different domains in tra2 protein were indentified. RT-PCR of the total RNA extracted from different tissue from the mosquitoes in different developmental stages was performed using specific primers. Results Two genes, namely Aaetra2-α and Aaetra2-β, were identified in different supercontig locations. The multi-transcripts were expressed by means of alternative promoters or terminators. The different domains in tra2 protein were defined as RS-rich N-terminal region, RNA recognition motif-RRM, linker region, and RS-rich C-terminal region. Both Aaetra2-α and Aaetra2-β showed sustained expression throughout the developmental stages of Ae.aegypti, and in all the tissues without a sex specificity. Conclusion Aaetra2 gene has multiple isoforms and is mapped to multiple locations in the genome. Aaetra2 has conservative functional domains of the sex-determining gene tra2. For Ae.agypti, Aaetra2 shows the potential as a new target for release of insects carrying a dominant lethal (RIDL) technology based on transgenic mosquitoes.
