Effects of parthenolide on high glucose-induced cell proliferation, NF-κB activation and MCP-1 expression in rat mesangial cells
- VernacularTitle:小白菊内酯对高糖诱导的大鼠肾小球系膜细胞增殖及核因子-κB、单核细胞趋化蛋白-1表达的影响
- Author:
Zhaozhong XU
1
;
Qianqian JIA
;
Hongyu LI
;
Jiancheng WANG
;
Yan ZHAO
;
Sijia CHEN
;
Haibo LONG
Author Information
1. 南方医科大学珠江医院急诊科
- Keywords:
parthenolide;
diabetic nephropathy;
nuclear factor kappa B;
monocyte chemoattractant protein-1
- From:
Journal of Southern Medical University
2013;(10):1471-1473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-κB activation and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs). Methods MCs were cultured in normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IκBα was detected by Western blotting to reflect NF-κB activity. EMSA method was used to measure the activation of NF-κB. Results MC proliferation, MCP-1 expression and NF-κB activation were significantly enhanced and the expression of NF-κB-binding protein IκBα was obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose. Conclusion PTL can suppress high glucose-stimulated NF-κB activation and MCP-1 expression in rat MC. These data provide a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.
