Effects of Xixin decoction on enzymes related to O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease
- VernacularTitle:洗心汤对SAD大鼠脑内tau蛋白O-GlcNAc糖基化修饰相关酶的影响
- Author:
Yongchang DIWU
1
;
Jinzhou TIAN
;
Jing SHI
Author Information
1. 陕西中医学院中医临床医学院
- Keywords:
sporadic Alzheimer's disease;
Xixin decoction;
O-GlcNAc transferase;
O-GlcNAc glycosidase
- From:
Journal of Southern Medical University
2013;(10):1442-1447
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Xixin decoction (XXD) on O-GlcNAc transferase (OGT) and O-GlcNAc glycosidase in O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease (SAD) and explore the possible mechanism. Methods Male SD rats were randomly divided into sham-operated group, model group, donepezil group, and low-, moderate-, and high-dose XXD groups. After treatment and behavioral test, the rats were sacrificed for detecting the expressions of OGT and O-GlcNAc glycosidase in the brain using immunohistochemistry and Western blotting. Results XXD significantly enhanced the expressions of OGT in the hippocampus of SAD rats and lowered the expression of O-GlcNAc glycosidase (P<0.05 or 0.01). OGT and O-GlcNAc glycosidase expressions showed no significant differences between the model group and donepezil group (P>0.05). Conclusion XXD can regulate the expression of OGT and O-GlcNAc glycosidase to enhance O-GlcNAc glycosylation of tau proteins in the hippocampus of SAD rats.