Diammonium glycyrrhizinate inhibits airway smooth muscle proliferation in a murine mod-el of chronic asthma
- VernacularTitle:甘草酸二铵抑制慢性哮喘模型小鼠气道平滑肌增生
- Author:
Min XIAO
1
;
Tao ZHU
;
Tao WANG
;
Fuqiang WEN
Author Information
1. 四川大学华西医院呼吸病学研究室/呼吸内科
- Keywords:
diammonium glycyrrhizinate;
asthma;
airway smooth muscle;
peroxisome proliferators-activated receptor gam-ma;
α-SMA
- From:
Journal of Southern Medical University
2013;(10):1416-1420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic value and possible mechanism of diammonium glycyrrhizinate (DG) in treatment of airway remodeling in a murine model of chronic asthma. Methods Thirty male BALB/C mice were randomly divided into control group, OVA+DG group and OVA group (n=10). HE staining was used to observe the pathological changes, and Masson's staining was used to detect and measure collagen deposition. Alpha-SMA and PPARγmRNA expressions were analyzed by RT-PCR, and the protein expressions ofα-SMA and PPARγwere measured by Western blotting. Results After 75 days of OVA sensitization and challenge, obvious pathological changes occurred in the lung tissues, which was more severe in OVA group than in OVA+DG group. Collagen deposition was significantly increased after OVA stimulation, but was obviously milder in OVA+DG group than in OVA group. OVA-induced up-regulation ofα-SMA was notably attenuated by DG injection. The expression of PPARγ was markedly down-regulated after OVA stimulation but was substantially enhanced after DG intervention. Conclusion DG can inhibit airway smooth muscle proliferation possibly through up-regulation of PPARγ in a murine model of chronic asthma.