Effect of erythropoietin on activities of antioxidant enzymes in the brain tissue of aged rats
10.3969/j.issn.1673-4254.2013.09.17
- VernacularTitle:促红细胞生成素对衰老大鼠脑组织中抗氧化酶活性的影响
- Author:
Yuefen ZHAI
1
;
Huiyun WANG
;
Huqing WANG
;
Hong SUN
;
Guilian ZHANG
;
Haiqin WU
Author Information
1. 西安交通大学医学院第二附属医院神经内科
- Keywords:
erythropoietin;
aging;
catalase;
glutathione peroxidase;
Nissl bodies
- From:
Journal of Southern Medical University
2013;(9):1332-1335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of erythropoietin (EPO) on the activities of antioxidant enzymes, namely catalase (CAT) and glutathione peroxidase (GSH-Px) in the brain tissues of aged rats. Methods Thirty SD rats were randomly divided into normal control, aging model, and recombinant human erthropoietin (rhEPO) treatment groups (n=10). Morris water maze was used to compare the behavioral indexes. The rats were then sacrificed to observe Nissl bodies in the hippocampal neurons with Nissl staining and test the activities of CAT and GSH-Px in the brain tissues. Results Compared with the control group, the aging rats showed significantly deteriorated learning and memory abilities (P<0.05), which were improved obviously by rhEPO treatment (P<0.05). The number of Nissl bodies in the neurons was reduced in the aging rats compared with that in the control group, and rhEPO treatment increased the number of Nissle bodies but failed to restore the control level. The aging rats also showed significantly lowered activities of CAT and GSH-Px in the brain tissue (P<0.05), which were increased significantly after rhEPO treatment (P<0.05). Conclusion EPO can enhance the activities of the antioxidant enzymes in the brain tissues of aged rats to increase the antioxidant capacity and produces an anti-aging effect.
