Expression of aquaporin-4 protein in the brain of preeclampsia model rats
10.3969/j.issn.1673-4254.2013.09.16
- VernacularTitle:水通道蛋白基因4在子痫前期模型大鼠脑组织中的表达
- Author:
Lei LIU
1
;
Huishu LIU
;
Qian HUANG
;
Yixin GAO
;
Bihui HU
Author Information
1. 广州医科大学附属广州市妇女儿童医疗中心
- Keywords:
preeclampsia;
eclampsia;
aquaporin-4;
brain edema;
animal models
- From:
Journal of Southern Medical University
2013;(9):1329-1331,1335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of aquaporin-4 (AQP4) protein in preeclampsia(PE) model rats. Methods Adult SD rats were divided into PE model group (n=7) induced by endotoxin, normal pregnancy group (n=6) and non-pregnancy group (n=12) treated with an equal volume of saline. AQP4 protein expression in the brain of the rats was detected with immunohistochemical staining and Western blotting. Results The blood pressure in PE model rats (135 ± 9 mmHg) was significantly higher than that in normal pregnancy rats (116 ± 8 mmHg) and non-pregnancy rats (112 ± 6 mmHg) (P<0.02). The rats in PE model group showed obvious proteinuria compared with the other two groups (3.8 ± 0.5 vs 2.6 ± 0.6 and 2.3 ± 0.4, P<0.05). Immunohistochemistry showed that AQP4 protein was localized primarily around the brain parenchymal blood vessels. Western analysis revealed a significantly elevated AQP4 protein expression in PE model group (39.6 ± 4.9) compared with that in normal pregnancy group (26.5 ± 4.3) and non-pregnancy group (9.7 ± 2.1) (P<0.01). Conclusion The up-regulation of AQP4 protein around the intraparenchymal blood vessels of the brain may play a role in the development of eclampsia.
