Role of miR-19a in ulcerative colitis in mice
10.3969/j.issn.1673-4254.2013.09.15
- VernacularTitle:miR-19a对溃疡性结肠炎的作用机制
- Author:
Pengzhi ZHOU
1
;
Bin CHEN
;
Pinjin HU
;
Yan SUN
Author Information
1. 中山大学附属第一医院消化科
- Keywords:
ulcerative colitis;
miR-19a;
tumor necrosis factor-α
- From:
Journal of Southern Medical University
2013;(9):1325-1328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of miR-19a in ulcerative colitis (UC) in mice. Methods The target gene of miR-19a was predicted by bioinformatics analysis. The expression of the target protein in UC colon was detected by immunohistochemistry and Western blotting. The target gene was further identified by enhanced green fluorescent protein (EGFP) report vector system. Results The target gene of miR-19a was TNF-αas predicted by bioinformatics analysis. TNF-αexpression was highly expressed in the colonic tissue of UC mice. MiR-19a could inhibit the report gene activity of TNF-α-3'UTR-WT but no that of TNF-α-3'UTR-Mut. Conclusion The target gene of miR-19a is TNF-α, and the binding site is TNF-α3'UTR. The possible role of miR-19a in UC pathogenesis involves regulation of TNF-αexpression in the colon.
