Detection of binding capability of targeted KDR ultrasound contrast agent in vitro for evaluating endometrial receptivity
10.3969/j.issn.1673-4254.2013.09.11
- VernacularTitle:血管内皮生长因子受体2单抗的靶向超声造影剂制备及体外评价
- Author:
Hongmei LIU
1
;
Xiaohua HAN
;
Li YANG
;
Lijing JI
;
Sushu LI
Author Information
1. 南方医科大学第三附属医院超声医学科
- Keywords:
targeted ultrasonic contrast agent;
KDR;
parallel plate flow chamber;
endometrial receptivity;
attachment
- From:
Journal of Southern Medical University
2013;(9):1308-1311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a new targeted liposome ultrasonic contrast agent with anti-KDR antibody that binds specifically with KDR as the main receptor of VEGF and evaluate its physical characteristics, biological activity and specific binding capability in vitro. Methods A sonicator was used to prepare the biotinylated lipid micro-bubbles (MB-B), and biotin-avidin-mediated technique was used for attachment of anti-mouse KDR monoclonal antibody to the micro-bubble shell to generate MB-BAB-KDR. MB-BAB-KDR was incubated with fluorescent second antibody to assess the link condition, and the control groups were the MB-B and micro-bubbles with the antibody alone (MB-B-KDR). A parallel plate flow chamber system was used to characterize micro-bubbles attachment efficiency to KDR Fc. Results The surface of the micro-bubbles could carry KDR antibody through the biotin-avidin bridge and MB-BAB-KDR were spherical and well-distributed. After incubation with the second antibody, MB-BAB-KDR could be observed to emit bright green fluorescence (Grade II) as compared with little or weak fluorescence in the control MB-B group (Grade 0) and MB-B-KDR group (Grade I). Targeted micro-bubbles bound to KDR Fc increased as the KDR Fc concentration increased (P<0.05). Conclusion The targeted liposome contrast agent linked with KDR antibody by biotin-avidin bridge we prepared shows an increased binding number as the KDR Fc concentration increases, which provides a novel approach to molecular imaging study of endometrial receptivity.
