Effect of the C-terminal truncated human apoptosis-inducing factorΔ1-480 on biological behaviors of MCF-7 cells
10.3969/j.issn.1673-4254.2013.09.06
- VernacularTitle:人凋亡诱导因子C-末端截短体AIFΔ1-480的表达对MCF-7细胞的影响
- Author:
Lijuan WANG
1
;
Huawei XIAO
;
E BAI
;
Xia ZHOU
;
Meng LI
;
Guihua JIN
;
Qing ZHU
Author Information
1. 西安交通大学医学院第一附属医院肿瘤中心肿瘤科
- Keywords:
C-terminal truncated human apoptosis-inducing factorΔ1-480;
MCF-7 cells;
proliferation;
apoptosis;
cytochrome c
- From:
Journal of Southern Medical University
2013;(9):1285-1288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of the C-terminal truncated human apoptosis-inducing factor (AIF) and its biological effect on MCF-7 cells. Methods PcDNA3.0-FDT-AIFΔ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamins. The expression of the truncated AIF gene was detected by Western blotting, and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c (cytC) were evaluated using flow cytometry, MTT assay, colony-forming assay, and mitochondrial membrane potential measurement. Results PcDNA3.0-FDT-AIFΔ1-480 enhanced AIF expression in MCF-7 cells, obviously inhibited the cell proliferation, and significantly reduced the mitochondrial membrane potentials (P<0.05). Transfection of the cells with PcDNA3.0-FDT-AIFΔ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells (P<0.05). Conclusion The expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.
