Expressions of APP,Fis1,PDCD7 and KLRF1 mRNA in acute myeloid leukemia
10.3321/j.issn:1673-4254.2009.11.039
- VernacularTitle:APP、Fis1、KLRF1和PDCD7基因在急性髓系白血病中的表达及临床意义
- Author:
Wei WANG
1
;
Fan-Yi MENG
;
Zou-Fang HUANG
;
Li LI
;
Yan-Xia CAI
;
Qi-Xin SUN
Author Information
1. 南方医科大学南方医院
- Keywords:
leukemia;
myeloid;
acute;
gene expression;
APP;
Fis1;
KLRF1;
PDCD7
- From:
Journal of Southern Medical University
2009;29(11):2259-2261,2265
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of amyloid precursor protein (APP), Fis1, PDCD7 and KLRF1 mRNA in acute myeloid leukemia (AML) and their prognostic significances. Methods Thirty-four AML patients and 10 patients with nonmalignant hematologic diseases (control group) were recruited in this study. Bone marrows were obtained from these patients to isolate the mononuclear cells, from which total RNA was extracted and reverse transcribed into cDNA. SYBR Green Ⅰ dye-based real-time quantitative PCR method was used to compare the expression of APP, Fis1, PDCD7 and KLRF1 mRNA between two groups. Results The expression level of PDCD7 mRNA in AML patients was significantly higher than that in the control group (Z=-2.213,P=0.027), but APP, Fisl and KLRF1 mRNA expression levels were similar between the two groups (P<0.05). The expression level of APP mRNA in M2 patients with t (8, 21) chromosomal abnormality was significantly higher than that in the control group (Z=-2.197, P=0.028), while APP mRNA expressions in M4b and M5b patients were both lower than those in the control group (Z=-2.368, P-0.018). The expression of APP mRNA differed significantly between the subtypes of AML, higher in M2 than in M4b and M5b (Z=-2.430, P=0.015; Z=-3.175, P=0.001, respectively). Conclusions The expression of PDCD7 mRNA increased significantly in AML patients, and APP mRNA expression is decreased in monocytic leukemia.
