Effects of different concentrations of amino acids in the culture medium on preimplantation mouse embryo development in vitro
10.3321/j.issn:1673-4254.2005.03.001
- VernacularTitle:氨基酸浓度对昆明小鼠胚胎体外发育的影响
- Author:
Xing DENG
1
;
Shuang WANG
;
Wu-Jian HUANG
;
Qiu-Zhen LIU
;
Wei-Bing XIE
;
Qing-Ling ZHANG
;
Wei-Yi FANG
;
Teng-Fei LIU
;
Chun HAN
;
Sha-Sha DU
;
Li-Sha WU
;
Yan-Qing DING
;
Kai-Tai YAO
Author Information
1. 南方医科大学
- Keywords:
mouse,Kunming;
preimplantation embryo;
development;
amino acid
- From:
Journal of Southern Medical University
2005;25(3):241-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of amino acids (AA) on the development of in vitro cultured preimplantation embryos of Kunming mice, and define the optimal AA concentration for embryo culture. Methods Totally 630 zygotes were collected from the oviducts of superovulated female Kunming mice, which were cultured in protein-free potassium simplex optimized medium (mKSOM) supplemented with Eagle's essential amino acids and Eagle's non-essential amino acids of different concentrations (mKSOM, mKSOM+1/16AA, mKSOM+1/8AA, mKSOM+1/4AA, mKSOM+1/2AA, mKSOM+AA, and mKSOM+2AA). Results The embryos cultured with the amino acids showed higher development rate to both 8-cell embryo stage and blastocyst stage than those cultured without amino acids. The correlation of amino acid concentration with 8-cell and blastocyst development rates conformed to the cubic model, with the highest development rate to both of the two stages observed at half of the amino acid concentration. Conclusion Amino acids can promote the development of preimplantation Kunming mouse embryos, but excessively high concentration of amino acids impair embryo development possibly because of metabolic and osmotic pressure changes of the embryos as well as toxicity of ammonium resulting from the metabolism of amino acids.
