Brain mitochondrial oxidative injuries induced by diazepam in mouse

VernacularTitle:安定导致小鼠大脑的线粒体氧应激损伤
Author: Xiao-Zhong QIU ¹ ; Yuan CHEN ; Mei ZHOU ; Wen-Zhu ZHANG
Author Information

1. First Military Medical University
From: Journal of Southern Medical University 2001;21(4):279-281
CountryChina
Language:Chinese
Abstract: Objective To probe into the molecular mechanisms by which a single dose of diazepam causes side-effect injuries to the brain. Mehtods A single dose of diazepam (10 mg/kg·b.w.) was injected daily into the abdominal cavity of the mice for 3 d. The mice receiving injection with saline served as control. The brain tissues weighing precisely 350 mg were obtained from each of the rats to isolate the mitochondria, and MnSOD and cytochrome C oxidase activities were determined. Assessment of the broken mitochondrial DNA was performed with FADU method. Results A single dose of diazepam caused the mitochondrial protein to decrease from (3.307±0.240) mg/ml to (2.7461±0.451) mg/ml (n=12, t=2.690, 0.01