Study of calcium-activated potassium channels regulation in hypothalamic neurons by NO- cGMP signal pathway

VernacularTitle:NO-cGMP通路对下丘脑钙激活钾通道的调控研究
Author: Shuai WANG ¹ ; Fei ZOU ; Hong-Bo GUO ; Chun-Qing CAI ; Bing-De LUO
Author Information

1. First Military Medical University
From: Journal of Southern Medical University 2001;21(4):263-265
CountryChina
Language:Chinese
Abstract: Objective To study the effects of nitric oxide on Ca2+-activated K+ (KCa) channels in the hypothalamus neurons of newborn SD rats. Methods The data were recorded using inside-out or cell-attached configuration of patch-clamp technique and the kinetic changes of KCa channels are compared before and after 100 μmol/L sodium nitroprusside (SNP) was added into the bathing solution. Results When 100 μmol/L SNP was added to the bathing solution in cell-attached configuration, the open probability of the KCa channels increased from (7.3±1.5)% to (40.2±6.5)%, open time from (7.12±1.41) ms to (15.34±3.45) ms, frequency from (11.3±3.5) Hz to (26.6±4.2) Hz. Conclusion NO-cGMP signal pathway could greatly increase the open probability of the channels as a result of both the prolongation of open period and increase of open frequency, but the pathological or physiological roles it may play require further study.