Construction of eukaryotic expression vectors for mouse anti-human fibrin scFv and low- molecular weight single-chain urokinase fusion gene
10.3321/j.issn:1673-4254.2001.04.007
- VernacularTitle:鼠抗人纤维蛋白单链抗体-低相对分子质量单链尿激酶融合基因真核分泌表达载体的构建
- Author:
Dong SHU
1
;
Bing XU
;
Wei-Yuan YU
;
Shen-Qiu LUO
Author Information
1. First Military Medical University
- From:
Journal of Southern Medical University
2001;21(4):261-262,281
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the eukaryotic expression vector for the fusion gene comprising the genes encoding mouse anti-human cross-linked fibrin single-chain fragment variable (scFv) antibody and low-molecular weight single-chain urokinase. Methods The single peptide of recombinant human ipro-urokinase was ligated with the target fusion gene, and the same reading frame was guaranteed. The ligated compound was subsequently incorporated respectively into pcDNA3 and pMJK expression vectors by DNA recombination technique. Results and Conclusion The desired recombinant plasmids pcDNA3 and pMJK3 containing the signal peptide of recombinant human ipro-urokinase gene and the fusion gene were constructed,which lays the foundation for establishing of a cell line that may secrete the fusion protein.
