Cloning and identification of human mannan-binding lectin- associated serine protease-2 gene
10.3321/j.issn:1673-4254.2001.04.003
- VernacularTitle:人甘露聚糖结合凝集素相关丝氨酸蛋白酶-2基因的克隆与鉴定
- Author:
Zheng-Liang CHEN
1
;
Li-Yun ZHANG
;
Gui-Lin QIAO
;
Xiao LU
Author Information
1. First Military Medical University
- From:
Journal of Southern Medical University
2001;21(4):248-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the gene fragment encoding human mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2). Methods The target cDNA fragment was amplified from the total RNA extracted from human fetal liver tissue by RT-nested PCR, cloned into pGEM-T vector and identified by restriction analysis and sequencing. Results The cDNA fragment encoding MASP-2 polypeptide with the signal sequence was isolated, linked with pGEM-T vector and transformed into E.coli TG1. The restriction maps of the selected clones were consistent with those generated by computer analyses. The result of sequencing of one selected clone showed that its sequence was identical with that of reported human MASP-2 cDNA. Conclusion The human MASP-2 gene was successfully cloned, which prepares the ground for studying the molecular mecha- nisms by which MBL activates the complement lectin pathway and the mutations of MBL gene cause immunodeficiency.
