Effects of loganin on inflammatory response and intestinal barrier damage in septic rats
- VernacularTitle:马钱苷对脓毒症大鼠炎症反应及肠道屏障损伤的影响
- Author:
Can WANG
1
;
Yantao LI
1
;
Zheng ZHOU
2
;
Lupeng WANG
3
;
Yuanyuan GAO
4
;
Shaoxi FAN
5
Author Information
1. Dept. of Critical Care Medicine，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China;Clinical Medical College，Hebei University of Chinese Medicine，Shijiazhuang 050011，China
2. Clinical Medical College，Hebei University of Chinese Medicine，Shijiazhuang 050011，China;Dept. of Pharmacy，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
3. Clinical Medical College，Hebei University of Chinese Medicine，Shijiazhuang 050011，China;Dept. of Vascular Surgery，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
4. Clinical Medical College，Hebei University of Chinese Medicine，Shijiazhuang 050011，China;Dept. of Obstetrics，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
5. Clinical Medical College，Hebei University of Chinese Medicine，Shijiazhuang 050011，China
- Publication Type:Journal Article
- Keywords:
loganin;
sepsis;
RhoA/ROCK1 signaling pathway;
inflammatory response;
intestinal barrier damage
- From:
China Pharmacy
2025;36(5):574-578
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of loganin on inflammatory response and intestinal barrier damage in septic rats by regulating the Ras homolog gene family member A （RhoA）/Rho-associated coiled-coil forming protein kinase 1 （ROCK1） signaling pathway. METHODS A sepsis rat model was established by cecal ligation and puncture， and randomly divided into sepsis group， loganin low-dose group （50 mg/kg loganin， gavage）， loganin high-dose group （200 mg/kg loganin， gavage）， positive control group （0.2 mg/kg atorvastatin， intraperitoneal injection）， and loganin high-dose + lysophosphatidic acid （LPA） group （200 mg/kg loganin gavage and intraperitoneal injection of 10 mg/kg RohA activator LPA）. An additional sham surgery group was established. Each group consisted of 10 rats， and medications were administered once every 6 hours for 4 times. After 24 hours of the last intervention， the levels of serum inflammatory factors interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α）， and IL-1β were detected. The pathological changes of ileal tissue were observed and Chiu’s intestinal mucosal injury score was also performed. The levels of intestinal function-lactate dehydrogenase （D-lactate）， D-amino acid oxidase （DAO） and endotoxin， the percentages of zonula occludens-1 protein （ZO-1） and Occludin positive staining area， as well as protein expressions of RhoA， and ROCK1 were all detected. com RESULTS Compared with the sepsis group， the percentages of ZO-1 and Occludin positive areas increased significantly in loganin low-dose and high-dose groups； while the levels of IL-6， TNF-α， IL-1β， DAO， D-lactate and endotoxin， Chiu’s intestinal mucosal injury score as well as protein expressions of RhoA and ROCK1 decreased significantly （P＜0.05）； the destruction of rat ileal tissue was alleviated， and tissue edema and inflammatory infiltration were significantly reduced； moreover， the improvement effect in loganin high-dose group was superior to that in loganin low-dose group （P＜0.05）. Compared with loganin high-dose group， RhoA activator LPA reversed the trend of changes in the above indicators （P＜0.05）. CONCLUSIONS Loganin can alleviate inflammatory response and intestinal barrier damage in septic rats， the mechanism of which may be associated with inhibiting RhoA/ROCK1 signaling pathway.
