Effects of loganin on inflammatory response and intestinal barrier damage in septic rats
- VernacularTitle:马钱苷对脓毒症大鼠炎症反应及肠道屏障损伤的影响
- Author:
Can WANG
1
,
2
;
Yantao LI
1
,
2
;
Zheng ZHOU
2
,
3
;
Lupeng WANG
2
,
4
;
Yuanyuan GAO
2
,
5
;
Shaoxi FAN
2
Author Information
1. Dept. of Critical Care Medicine，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
2. Clinical Medical College，Hebei University of Chinese Medicine，Shijiazhuang 050011，China
3. Dept. of Pharmacy，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
4. Dept. of Vascular Surgery，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
5. Dept. of Obstetrics，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
- Publication Type:Journal Article
- Keywords:
loganin;
sepsis;
RhoA/ROCK1 signaling pathway;
inflammatory response;
intestinal barrier damage
- From:
China Pharmacy
2025;36(5):574-578
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of loganin on inflammatory response and intestinal barrier damage in septic rats by regulating the Ras homolog gene family member A （RhoA）/Rho-associated coiled-coil forming protein kinase 1 （ROCK1） signaling pathway. METHODS A sepsis rat model was established by cecal ligation and puncture， and randomly divided into sepsis group， loganin low-dose group （50 mg/kg loganin， gavage）， loganin high-dose group （200 mg/kg loganin， gavage）， positive control group （0.2 mg/kg atorvastatin， intraperitoneal injection）， and loganin high-dose + lysophosphatidic acid （LPA） group （200 mg/kg loganin gavage and intraperitoneal injection of 10 mg/kg RohA activator LPA）. An additional sham surgery group was established. Each group consisted of 10 rats， and medications were administered once every 6 hours for 4 times. After 24 hours of the last intervention， the levels of serum inflammatory factors interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α）， and IL-1β were detected. The pathological changes of ileal tissue were observed and Chiu’s intestinal mucosal injury score was also performed. The levels of intestinal function-lactate dehydrogenase （D-lactate）， D-amino acid oxidase （DAO） and endotoxin， the percentages of zonula occludens-1 protein （ZO-1） and Occludin positive staining area， as well as protein expressions of RhoA， and ROCK1 were all detected. com RESULTS Compared with the sepsis group， the percentages of ZO-1 and Occludin positive areas increased significantly in loganin low-dose and high-dose groups； while the levels of IL-6， TNF-α， IL-1β， DAO， D-lactate and endotoxin， Chiu’s intestinal mucosal injury score as well as protein expressions of RhoA and ROCK1 decreased significantly （P＜0.05）； the destruction of rat ileal tissue was alleviated， and tissue edema and inflammatory infiltration were significantly reduced； moreover， the improvement effect in loganin high-dose group was superior to that in loganin low-dose group （P＜0.05）. Compared with loganin high-dose group， RhoA activator LPA reversed the trend of changes in the above indicators （P＜0.05）. CONCLUSIONS Loganin can alleviate inflammatory response and intestinal barrier damage in septic rats， the mechanism of which may be associated with inhibiting RhoA/ROCK1 signaling pathway.
