Study on the effect of slightly acid hypochlorous water on dental unit waterlines biofilm model
10.3760/cma.j.cn112150-20210329-00310
- VernacularTitle:微酸性次氯酸水对口腔综合治疗台水路模拟生物膜作用效果的研究
- Author:
Yaguang CHENG
1
;
Pengju XIN
;
Jing SU
Author Information
1. 首都医科大学附属北京口腔医院老年科，北京 100050
- Keywords:
Biofilm;
Disinfection;
Dental equipment;
Waater pollution;
Innfection control
- From:
Chinese Journal of Preventive Medicine
2021;55(10):1245-1249
- CountryChina
- Language:Chinese
-
Abstract:
Pseudomonas aeruginosa biofilm was established on PU and PVC′s surface by Center of Disease Control (CDC) Biofilm Reactor, to evaluate the effectiveness of Slightly Acidic Hypochlorous water (SAHW) for removing Pseudomonas aeruginosa biofilm on Polyurethane (PU) or Polyvinyl Chloride (PVC), which are commonly used in dental unit waterlines (DUWL). The biofilms were treated with 10 mg/L SAHW, 40 mg/L SAHW and sterile distilled water (control group) by the continuous immersion method for 3, 7, and 10 days. The total viable count (TVC) and Confocal laser scanning microscopy (CLSM) were selected to evaluate the biofilm removal effect and calculate the biofilm reduction rate. The results of TVC showed that 99%-100% biofilm reduction could be achieved in 10 mg/L SAHW group and 40 mg/L SAHW group. The results of CLSM showed that 10 mg/L SAHW group and 40 mg/L SAHW group could reach 89%-100% biofilm reduction after 10 days of treatment.
