Progress in preclinical research on induced pluripotent stem cell therapy for acute myocardial infarction
10.3724/zdxbyxb-2023-0402
- VernacularTitle:基于诱导多能干细胞治疗急性心肌梗死的临床前研究进展
- Author:
Songyan CAI
1
;
Qingyuan DAI
Author Information
1. 昆明医科大学第一附属医院心内科,云南 昆明 650032
- Keywords:
Induced pluripotent stem cells;
Cardiac muscle cells;
Acute myocardial infarction;
Regenerative medicine;
Review
- From:
Journal of Zhejiang University. Medical sciences
2024;53(2):244-253
- CountryChina
- Language:Chinese
-
Abstract:
Induced pluripotent stem cells(iPSCs)are obtained by introducing exogenous genes or adding chemicals to the culture medium to induce somatic cell differentiation.Similarly to embryonic stem cells,iPSCs have the ability to differentiate into all three embryonic cell lines.iPSCs can differentiate into cardiac muscle cells through two-dimensional differentiation methods such as monolayer cell culture and co-culture,or through embryoid body and scaffold-based three-dimensional differentiation methods.In addition,the process of iPSCs differentiation into cardiac muscle cells also requires activation or inhibition of specific signaling pathways,such as Wnt,BMP,Notch signaling pathways to mimic the development of the heart in vivo.In recent years,suspension culturing in bioreactors has been shown to produce large number of iPSCs derived cardiac muscle cells(iPSC-CMs).Before transplantation,it is necessary to purify iPSC-CMs through metabolic regulation or cell sorting to eliminate undifferentiated iPSCs,which may lead to teratoma formation.The transplantation methods for iPSC-CMs are mainly injection of cell suspension and transplantation of cell patches into the infarcted myocardium.Animal studies have shown that transplantation of iPSC-CMs into the infarcted myocardium can improve cardiac function.This article reviews the progress in preclinical studies on iPSC-CMs therapy for acute myocardial infarction and discusses the limitations and challenges of its clinical application to provide references for further clinical research and application.
