The first outbreak of dengue fever and molecular tracing in Puyang, 2019
10.3760/cma.j.cn112150-20200818-01131
- VernacularTitle:2019年濮阳市登革热疫情与病原分子溯源
- Author:
Guofeng XU
1
;
Qingjie ZHANG
;
Yuhua ZHANG
;
Xiaofei MENG
;
Guangkang LI
;
Yonghao GUO
;
Weimin XIE
;
Shengli XIA
Author Information
1. 濮阳市疾病预防控制中心微生物检验科 457000
- Keywords:
Dengue virus;
Genotype;
Diagnosis;
Molecular tracing
- From:
Chinese Journal of Preventive Medicine
2021;55(8):978-982
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study retrospectively analyzed an outbreak of dengue fever in Puyang of Henan province in 2019, in order to find the sources of infection.Methods:Dengue virus IgM/IgG and NS1 antigen were tested by colloidal gold method. E gene was amplified by PCR. MegaX was used for sequences alignment to construct evolutionary distance trees.Results:After clinical and laboratory confirmation, there were 81 cases of dengue fever, 17 of which were imported case who were local farmers and worked in Combadia and Thailand, and 64 of which were indigenous cases. The E gene alignment results showed that the pathogen of this epidemic was Vietnamese 1 and highly homologous with the Vietnamese strain. After the local outbreak, dengue virus E gene developed a nucleotide site mutation which can be steadily transmission.Conclusion:The dengue fever outbreak in Puyang was a local outbreak caused by dengue virus type 1, which was associated with imported cases. Gene sequencing showed that the imported pathogen had a relatively stable and transmissible nucleotide mutation after the local epidemic.
