Preparation, characterization and cytology study of Pluronic-PEI micelles
10.3785/j.issn.1008-9292.2017.04.03
- VernacularTitle:普朗尼克—聚乙烯亚胺纳米胶束的制备及其细胞生物学特性
- Author:
Hebin WANG
1
;
Yang LI
;
Xingang LIU
;
Jun ZHOU
;
Qingqing WANG
;
Guping TANG
Author Information
1. 新疆兵团南疆化工资源利用工程实验室塔里木大学生命科学学院
- Keywords:
Technology,pharmaceutical;
Polyethyleneimine/chemical synthesis;
Nanospheres;
Drug carriers;
Genes;
Transfection;
Adenosine triphosphate;
P-glycoprotein;
Drug resistance,neoplasm
- From:
Journal of Zhejiang University. Medical sciences
2017;46(2):125-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare and characterize Pluronic-PEI micelles as a drug/ gene delivery system.Methods:The low-molecular-weight PEI was used as a cross-linking agent to prepare Pluronic-PEI micelles.The particle size,zeta potential and critical micelle concentration (CMC) were measured by dynamic light scattering (DLS) and pyrene fluorescence probe.The cytotoxicity,transfection efficiency and the impact on the intracellular ATP and P-gp levels of Pluronic-PEI micelles were investigated at the cellular level.Results:Pluronic-PEI micelles were successfully prepared with a suitable particle size (120-180 nm),zeta potential (+ 6-+ 9 mV),and a good ability to carry the drug/gene.An in-vitro study showed that Pluronic-PEI had low cytotoxicity,and the P123-PEI600 possessed high gene transfection efficiency and could downregulate the intracellular ATP and P-gp levels.Conclusion:Pluronic-PEI is a good drug/gene delivery system,and P123-PEI600 is an ideal vector,which may be used in the combination therapy for reversing multidrug resistance.
