T cell erceptor β-chain CDR3 spectratyping and cytom egalovirus activation in allogeneic hematopoietic stem cell transplant rceipients
10.3785/j.issn.1008-9292.2016.09.10
- VernacularTitle:异基因造血干细胞移植受者T细胞受体β链CDR3谱型表达与巨细胞病毒激活
- Author:
Zhihua WU
1
;
Min JING
;
Hanying LIANG
;
Rong YANG
;
Yaping HUANG
;
Xiaoming CHEN
;
Jianhua HU
;
Jun FAN
Author Information
1. 浙江大学医学院附属第一医院传染病诊治国家重点实验室
- Keywords:
Hematopoietic stem cell transplantation;
Genes,T-cell receptor beta/immunology;
Complementarity determining regions/immunology;
Gene expression profiling;
Cytomegalovirus;
Polymerase chain reaction
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):514-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the association between T-cell receptor beta variable (TCR BV) complementarity determining region 3 (CDR3) spectratyping and CMV activation in the recipients of allogeneic hematopoietic stem cell transplantation (HSCT).Methods:Fluorescence quantitative PCR melting curve analysis was used to sequence 24 TCR BV families in 7 HSCT recipients and 3 healthy controls .CMV-pp65 antigenemia was measured by immunohistochemical staining .Plasma IgM specific for CMV was identified using ELISA .Relationship between TCR BV families and CMV activation was statistically analyzed .Results: Twenty-four TCR BV families were expressed in 3 healthy controls, while TCR BV CDR3 sequencing results in 7 recipients turned out to be BV9, BV11, BV17, BV20 and so on.Amino acid sequence features were as follows: TCR BV9 contained “QVRGGTDTQ”, TCR BV11 contained“VATDEQ” and “LGDEQ”, TCR BV17 contained “IGQGNTEA”, and TCR BV20 contained “VGLAANEQ”.Five recipients suffered from pp65 antigenemia in 3 month after transplantation , and pp65-positive cells ranged from 2 to 15 per 5 ×104 white blood cells.Three recipients were CMV-IgM positive.No significant differences were found in TCR BV families between pp 65-positive recipients and pp65-negative recipients ( all P>0.05 ) .But there was statistically significant difference in frequency of TCR BV11 between CMV-IgM negative recipients and CMV-IgM positive recipients ( P<0.05 ) .Conclusion: T cell immune response was characterized by special TCR BV CDR3 spectratyping in HSCT recipients , and TCR BV11 expression may be associated with CMV activation .
