Effect of methyleugenol on expression of MUC5 AC in nasal mucosa of rats with allergic rhinitis
10.3785/j.issn.1008-9292.2016.09.05
- VernacularTitle:甲基丁香酚对变应性鼻炎大鼠鼻黏膜组织中黏蛋白5AC的影响
- Author:
Nannan MENG
1
;
Yun HOU
;
Yan GUI
;
Kehu XI
;
Youhu WANG
;
Jing YANG
;
Hong CHEN
;
Xiaobing ZHANG
Author Information
1. 兰州大学第一医院儿童保健中心
- Keywords:
Eugenol/pharmacology;
Eugenol/administration & dosage;
Rhinitis/drug therapy;
Loratadine/therapeutic use;
Mucins/drug effects;
Nasal mucosa/drug effects;
Rats,Wistar
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):476-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of methyleugenol on expression of MUC5AC in nasal mucosa of rats with allergic rhinitis (AR).Methods: Seventy-two Wistar rats were randomly divided into 6 groups: normal control group , AR group , loratadine group , low-dose methyleugenol group , middle-dose methyleugenol group and high-dose methyleugenol group with 12 rats in each group . AR was induced by intraperitoneal injection of ovalbumin in latter 5 groups .10 mg loratadine q .d was given to rats in loratadine group by gavage; and 10 mg/kg, 20 mg/kg and 40 mg/kg methyleugenol were given by gavege q .d to rats in low-, middle-and high-dose methyleugenol groups , respectively .Nasal mucosa samples were obtained from rats at 1, 2, 4 and 6 weeks after drug intervention .The expression of MUC5AC protein and mRNA in nasal mucosa was detected by immunohistochemistry and real-time fluorescence quota PCR ( RT-PCR ) , respectively .Results: Compared with AR , the percentage of cells staining positively for MUC 5AC protein and the relative quantity of MUC5AC mRNA in middle-and high-dose methyleugenol groups were significantly decreased after 2 and 4 weeks of drug intervention ( P<0.05 ) , but no such decrease was observed in low-dose methyleugenol group at all time points ( P >0.05 ) .The percentage of cells with positive expression of MUC 5 AC protein and mRNA in loratadine group were significantly decreased after 1 week of administration ( P <0.05 ) .The percentage of cells with positive MUC 5 AC protein in middle-dose methyleugenol group was higher than that in loratadine group ( P<0.05 ) after 6 week of drug intervention , but the difference was not seen in high-dose group ( P >0.05 ) . There was no significant difference in relative quantities of MUC 5 AC mRNA after 4 weeks of administration between high-and middle-dose methyeugenol groups and loratadine group ( P >0.05 ) . Conclusion:Methyleugenol can attenuate AR through inhibiting the expression of MUC5AC mRNA and protein in nasal mucosa of AR rats .
