Effect and its molecular mechanisms of curcumin on pulmonary artery smooth muscle cells in rat model with chronic obstructive pulmona ry disease
10.3785/j.issn.1008-9292.2016.09.04
- VernacularTitle:姜黄素对慢性阻塞性肺疾病大鼠肺动脉平滑肌细胞的作用及其机制研究
- Author:
Xiangang LIN
1
;
Yenong CHEN
;
Zhuqing LIU
Author Information
1. 安徽中医药大学针灸骨伤临床学院
- Keywords:
Curcumin/administration & dosage;
Curcumin/pharmacology;
Pulmonary disease,chronic obstructive/drug therapy;
Pulmonary disease,chronic obstructive/physiopathology;
Pulmonary artery/cytology;
Muscle,smooth,vascular /drug effects;
Disease models,animal
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):468-475
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects and the underlying molecular mechanisms of curcumin on pulmonary artery smooth muscle cells in rat model with chronic obstructive pulmonary disease (COPD).Methods: A total of 75 male Wistar rats were randomly divided into control group (group CN), model group (group M), low-dose curcumin group (group CL), medium-dose curcumin group(group CM) and high-dose curcumin group ( group CH ) . HE staining was used to observe the morphology of pulmonary artery .Proliferating cell nuclear antigen ( PCNA) , apoptosis-related protein Bcl-2 and Bax were detected by immunohistochemical staining .TUNEL kit was used to analyze the effects of curcumin on apoptosis of smooth muscle cells , and the protein expressions of SOCS-3/JAK2/STAT pathway in lung tissues were determined by western blot . Results: Right ventricular systolic pressure ( RVSP ) and right ventricular hypertrophy index ( RVMI) in group M were significantly higher than those in group CN , group CH and group CM ( all P<0.05 ) .HE staining and TUNEL kit test showed that the number of pulmonary artery smooth muscle cells had a significant increase in group M , while the pulmonary artery tube became thin , and the smooth muscle cells shrinked in group CM and group CH .Immunohistochemistry showed that PCNA and Bcl-2 in group M were significantly higher than those in group CN ( all P<0.05 ) , while Bax expression was significantly lower than that in group CN ( P <0.05 ) .PCNA in group CM and group CH were significantly lower than that in group M ( all P<0.05 ) , while Bax expression was significantly higher than that in group M ( P<0.05 ) .Western blot showed that SOCS-3 protein was significantly decreased in group M, while the p-JAK2, p-STAT1, p-STAT 3 were significantly increased (all P<0.05 ) .Compared with group M , SOCS-3 protein in group CM and group CH were significantly increased ( all P<0.05 ) , while the p-JAK2 , p-STAT3 were significantly reduced ( all P<0.05 ) .Conclusion:Curcumin could promote the apoptosis of smooth muscle cells in rats with COPD , and improve the mean pulmonary artery pressure and RVMI through stimulating SOCS-3/JAK2/STAT signaling pathway .
