Protective effect of diosgenin on chondrocytes mediated by JAK2/STAT3 signaling pathway in mice with osteoarthritis
10.3785/j.issn.1008-9292.2016.09.02
- VernacularTitle:JAK2／STAT3信号通路介导薯蓣皂苷元对骨性关节炎软骨细胞代谢的影响
- Author:
Jun LIU
1
;
Xiaole HE
;
Ping ZHEN
;
Shenghu ZHOU
;
Xusheng LI
Author Information
1. 兰州军区兰州总医院全军骨科中心关节外科
- Keywords:
Osteoarthritis/pathology;
Dioscin/pharmacology;
STAT3 transcription factor;
Protein-tyrosine kinases;
Chondrocytes/pathology;
Oxidative stress
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):452-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of diosgenin ( Dgn) on chondrocytes and its relation to JAK2/STAT3 signaling pathway in mice with osteoarthritis ( OA). Methods: Fifteen male C57BL/6 mice were randomly divided into three groups:control group , OA group and OA +Dgn group . After 4 weeks of treatment , the histopathological changes of cartilage tissue were observed by toluidine blue staining under light microscopy and the ultrastructure of chondrocytes was observed under electron microscopy .The primarily cultured chondrocytes of OA mice were randomly divided into 4 groups: (1) OA group, (2) Dgn group, (3) Dgn +AG490 group, (4) AG490 group. The expression of p-JAK2, p-STAT3, Bax, succinate dehydrogenase ( SDH ) and cytochrome c oxidase ( COX ) were detected by Western blotting, and superoxide dismutase ( SOD) was detected using colorimetric method . Results: The morphological observation showed that the chondrocytes of OA group presented considerable pathological changes , while the chondrocytes in OA+Dgn group maintained intact membrane .Electron microscopy observation found obvious injury in cartilage tissues of OA group , while that in OA +Dgn group remained smooth . Compared with OA group , the expressions of p-JAK2 and p-STAT3 in chondrocytes of Dgn group were increased (all P<0.05), and the expressions of Bax protein , SDH, COX and SOD were decreased ( all P<0 .05 ) .While compared with Dgn group , the expressions of p-JAK2, p-STAT3, SDH, COX and SOD in chondrocytes of Dgn +AG490 group were decreased (all P<0.05), and the expression of Bax protein was increased ( P <0.05 ).Conclusion: Diosgenin can inhibit apoptosis and increase mitochondrial oxidative stress capacity of chondrocytes in mice with osteoarthritis , which is closely related to the activation of JAK 2/STAT3 signaling pathway .
