Inhibitory effect of suramin on inflammatory response in pulmonary tissue and peripheral blood in LPS-induced septic mice
10.3785/j.issn.1008-9292.2015.09.13
- VernacularTitle:苏拉明对脓毒症小鼠肺组织和循环炎症反应的抑制作用
- Author:
Liang HAN
1
;
Jin-Chao HOU
;
Xiang-Ming FANG
Author Information
1. 浙江大学医学院附属第一医院麻醉科
- Keywords:
Suramin/pharmacology;
Lung/injuries;
Sepsis/drug therapy;
Tumor necrosis factor-alpha;
Interleukin-6;
NF-kappa B;
Disease models,animal
- From:
Journal of Zhejiang University. Medical sciences
2015;(5):553-558
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of suramin on inflammatory response in pulmonary tissue and peripheral blood in septic mice .Methods: Twenty-four male C57 BL/6 mice were randomly divided into two groups , and suramin ( 5 mg/kg ) or normal saline was intravenously injected 30 min before LPS ( 5 mg/kg ) infusion , respectively .The contents of TNF-αand IL-6 in pulmonary tissue and peripheral blood were detected by ELISA .Suramin or saline-pretreated human mononuclear THP-1 cells were treated with 100 ng/mL LPS in vitro.The expression of TNF-αand IL-6 mRNA and the activity of NF-κB were analyzed by quantitative PCR and Western blotting at different time points after LPS treatment , respectively.Results: Compared with the saline group, the TNF-αand IL-6 levels in pulmonary tissue and peripheral blood were significantly reduced in suramin group at 24 h after LPS treatment ( all P <0 .01 );while there was no significant difference at 72 h between two groups ( all P>0 .05 ) . The expression of TNF-α, IL-6 mRNA and the activity of NF-κB was decreased in suramin group at different time points after LPS treatment .Conclusion: Suramin can protect LPS-induced acute lung injury through down-regulation of systemic and pulmonary pro-inflammatory factors , which may be associated with the inhibition of NF-κB activity.
